TEXTBOOK:    Benson’s Microbiological Applications 10^th edition, 2007 Laboratory Manual in General Microbiology by Alfred E.     Brown

EXAMS/REPORTS/HOMEWORK PROJECT:

LAB EXAM # 1    = 75 PTS    FEB 19, 2008

LAB EXAM # 2    = 75 PTS   MAR 18, 2008

LAB EXAM # 3    = 75 PTS   APR 22, 2008 

FINAL LAB  PRESENTATION  = 50 PTS

12 @ 20PTS LAB REPORTS = 240 PTS

Lab Report format is on my WEBSITE and it is included as part of this syllabus, so that you may download it.

All lab reports must follow this format and will lose 5 points for lateness.

GRADE SCALE:          A = 90 -100 %

B = 80 - 89 %

C = 70 - 79 %

D = 60 - 69 %

F = < 60 %

IMPORTANT DEADLINES:

LAST DAY FOR CREDIT/NO CREDIT:  MAR 20, 2008 

LAST DAY FOR WITHDRAWAL WITHOUT W:  FEB 1, 2008

LAST DAY FOR WITHDRAWAL WITH W:  MAR 20, 2008

FINAL PRESENTATION DATE/TIME:  TUESDAY MAY 6, 2008 @ 3:00 - 5:30 pm 3393-103  

COURSE OBJECTIVES:  This course will provide hands on experience in the survey of Microbiology.

Students will learn about rules and regulations for dealing with microorganisms, aseptic technique methodology, safe use of chemical agents, microscopes and other instruments.

Students will practice calculations, learn how to analyze and discuss their own hand on experimental data and prepare laboratory reports.

STUDENT LEARNING OUTCOMES: After completion of Microbiology laboratory the student should be able to:

1)      Demonstrate the ability to use and understand the Scientific Method of Reasoning and apply it to their daily lives.

2)     List procedural steps on carrying out their experiments and construct an organized, formal lab report.

3)     Use microscopic observation and computer microscopy to identify and classify both macroscopic and microscopic organisms, and their corresponding anatomical structures.

4)     Demonstrate proper aseptic techniques to manipulate microorganisms and promote a healthy and safe laboratory environment.

5)     Exemplify the classification principles of Domains and Kingdoms, and the ubiquitous nature of Micro-organisms.

POLICIES AND PROCEDURES:

Follow Biosafety Rules and Regulations

All lab participants will need to purchase and bring a Lab Coat to class. The lab coat will remain in the lab, do not show up in scrubs as you cannot leave them in lab (unless you have an extra layer of clothing)

Always where closed toed shoes in the laboratory. If you are wearing open toes, “slippahs” etc, you may not participate.

No eating any foods or snacks, chewing gum, or drinking fluids in the laboratory.

If you have  “latex” allergy, please let me know as you may need to provide your own disposable gloves.

Lab Report format is on WEBSITE and it is included in this syllabus, so that you may download it.

All lab reports must follow this format and will lose 5 points/week for lateness. 

No Make-up for missing lab exercises!. (Lab reports are equal to 20 points, therefore you will

receive a 0 for that particular lab)

No Make-up for missing Lab Exams or Final Presentation! 

FALL 2007    GENERAL MICROBIOLOGY 130 LABORATORY (16023)

TUESDAY  3:00 - 5:45   MANONO CAMPUS BLDG 3393 - RM 103

LAB #                          DATE                        TOPIC                          Lab Manual pages       

   1          JAN 15     Introduction, Lab Safety, Microorganisms and ix-xii, Exp #1, p 1-11

                               Pathogenicity, Brightfield Microscopy

   2            JAN 22  Microscope Measurements                                    Exp # 4, p 25-29

                               Metric System and Scientific Notation

   3          JAN 29    Survey of Microbes: Pond Water                          Exp # 5, p 31-46

                               Protozoa, Algae and Cyanobacteria

   4          FEB 5        The Fungi: Yeasts and Molds    -set up                   Exp # 7, p 53-60  

                               Aseptic Technique Methods                                  Exp # 8, p 63-72

                               Culture Media Preparation (discussion)                  Exp #18, p 123-132

   5          FEB 12      The Fungi: Yeasts and Molds    - Results                Exp # 7, p 53-60       

   6          FEB 19      LAB EXAM # 1, TURN IN LAB REPORTS

                               Ubiquity of Bacteria -set up                                 Exp # 6, p 47-52

   7          FEB 26      Ubiquity of Bacteria -Results                               Exp # 6, p 47-52

                               Smear Preparation                                                Exp # 10, p 87-90

                               Simple Stain                                                        Exp # 11, p  91-94

   8          MAR   4    Pure Culture Techniques - setup                            Exp #  9, p 73-83                                       

   9                          Capsule Stain, Flagella Stain                                 Exp # 15, 16

   8          MAR 11     Pure Culture Techniques -Results                          Exp #  9, p 73-83        

                               Gram Staining,                                                      Exp # 14, p 101-104

              MAR 18     LAB EXAM # 2, TURN IN LAB REPORTS

                               Enumeration of Bacteria: The Standard Plate         Exp # 20, p139-147

                               Count- Simulation and review   

              MAR 25     SPRING BREAK- NO CLASSES

   10      APR  1        Anti-microbic Sensitivity Testing: Kirby Bauer

                               Method –setup                                                     Exp # 33, p 211-216

                               Staphylococci: Isolation                                       Exp # 52

   11        APR 8        Anti-microbic Sensitivity Testing: Kirby Bauer     Exp # 33, p 211-216

                               Staphylococci: Identification                               Exp #52

   12       APR 15       UV Sensitivity set-up;   Work on Unknown

   12       APR 22      LAB EXAM # 2, TURN IN LAB REPORTS

                      UV Sensitivity results;   Work on Unknown

             APR 29       Work on Unknown/ Prepare for Class Presentations

     MAY 6, 2008 LAB FINAL PRESENTATIONS AND TURN IN LAB REPORTS AND

                             FINAL PRESENTAION PAPER OR POWERPOINT 

LAB REPORTS MUST USE THE FOLLOWING FORMAT

                               (TEMPLATE FOR ALL LAB REPORTS)

                  PLEASE CHANGE  LAB #’s, DATES AND LAB TITLES

LAB #:

RESEARCHER:

DATE:

TITLE:

HYPOTHESIS:

MATERIALS: (list all materials needed in vertical columns format)

METHODS: (Summarize all procedures)

RESULTS: (Include all labeled drawings, any question and answer  assignments from each lab, and all data collected.)

DISCUSSION AND CONCLUSIONS

**ALL LAB REPORTS MUST BE TYPED AND TURNED IN ON TIME.

EACH STUDENT MUST SUBMIT HIS/HER OWN LAB REPORT

PLEASE USE YOUR OWN WORDS, DO NOT COPY SECTIONS FROM

TEXTBOOKS OR PLAGERIZE OTHER STUDENTS WORK. (I READ THEM!)

ALL LAB REPORTS ARE DUE ON THE DAY OF THE EXAM.

LATE LAB REPORTS WILL LOSE 5 POINTS FOR EACH WEEK THAT THEY ARE LATE!

ATTENDANCE WILL BE TAKEN ON LAB DAYS…

MISSED LAB EXERCISES WILL RECEIVE 0 POINTS, YOU MAY WANT TO LOOK AT SOMEONE ELSE’S DATA BUT DO NOT TURN THE REPORT IN)